Second Derivative

Features:

• Generate the second derivative of the spectra.

• Second derivative spectroscopy is a technique that enhances the separation of overlapping peaks. The second derivative measures the instantaneous rate of change of the first derivative. The sign of the second derivative tells us whether the slope of the tangent line is increasing or decreasing.

Steps:

1.       Load the file. Select Spectra Mathematics and select Second Derivative from the dropdown menu.

2.      Click Select 1st spectrum. Draw a region of interest. A spectrum corresponding to the average spectrum of the selected region will appear in the SPECTRAL ANALYSIS panel.

3.      Press Generate and observe a new spectrum.  

Tip: You can save the spectra using the brushing mode available from the Strip Toolbar.

Note: You can also execute the second derivative of the entire dataset by selecting Second Derivative from Image Algebra available from the DATACUBE MATHEMATICS panel.